Application
Western blot analysis of APOE(arrow) using rabbit polyclonal APOE Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the APOE gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with APOE antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | APOE Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APOE; Apolipoprotein E |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | APOE Apolipoprotein E |
|---|---|
| Molecular Weight(MW) | 36154 Da |
| Function | Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues |
| Tissue Specificity | Occurs in all lipoprotein fractions in plasma. It constitutes 10-20% of very low density lipoproteins (VLDL) and 1-2% of high density lipoproteins (HDL). APOE is produced in most organs. Significant quantities are produced in liver, brain, spleen, lung, adrenal, ovary, kidney and muscle |
| Cellular Localization | Secreted. |
| Entrez Gene | 348 |
|---|
Application
Western blot analysis of APOE(arrow) using rabbit polyclonal APOE Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the APOE gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with APOE antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
