Application
Western blot analysis of APOL2 Antibody (Center) in HepG2 cell line lysates (35ug/lane). APOL2 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with APOL2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
APOL2 Antibody (Center) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of APOL2 (arrow) using rabbit polyclonal APOL2 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APOL2 gene.Application
Confocal immunofluorescent analysis of APOL2 Antibody (Center)(Cat#AP8848c) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | APOL2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APOL2; Apolipoprotein L2; Apolipoprotein L-II |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:100-500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | APOL2 Apolipoprotein L2 Apolipoprotein L-II |
|---|---|
| Molecular Weight(MW) | 37092 Da |
| Function | May affect the movement of lipids in the cytoplasm or allow the binding of lipids to organelles |
| Tissue Specificity | Widely expressed; the highest levels are found in lung, thymus, pancreas, placenta, adult brain and prostate; also detected in spleen, liver, kidney, colon, small intestine, uterus, spinal cord, adrenal gland, salivary gland, trachea, mammary gland, skeletal muscle, testis and fetal brain and liver |
| Cellular Localization | Cytoplasm (Probable). |
| Entrez Gene | 23780 |
|---|
Application
Western blot analysis of APOL2 Antibody (Center) in HepG2 cell line lysates (35ug/lane). APOL2 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with APOL2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
APOL2 Antibody (Center) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of APOL2 (arrow) using rabbit polyclonal APOL2 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APOL2 gene.
Application
Confocal immunofluorescent analysis of APOL2 Antibody (Center)(Cat#AP8848c) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100-500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
