Application
Western blot analysis of APRT (arrow) using rabbit polyclonal APRT Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the APRT gene (Lane 2) (Origene Technologies).Application
Western blot analysis of APRT Antibody (N-term) in A2058(lane 1),MCF-7(lane 2) cell line lysates (35ug/lane). APRT (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with APRT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of HepG2 cells using APRT Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | APRT Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APRT; Adenine phosphoribosyltransferase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | APRT Adenine phosphoribosyltransferase |
|---|---|
| Molecular Weight(MW) | 19608 Da |
| Function | Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis |
| Tissue Specificity | This APRT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19~48 amino acids from the N-terminal region of human APRT. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 353 |
|---|
Application
Western blot analysis of APRT (arrow) using rabbit polyclonal APRT Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the APRT gene (Lane 2) (Origene Technologies).
Application
Western blot analysis of APRT Antibody (N-term) in A2058(lane 1),MCF-7(lane 2) cell line lysates (35ug/lane). APRT (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with APRT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of HepG2 cells using APRT Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
