Application
Western blot analysis of APTX Antibody (Center) in HepG2 cell line lysates (35ug/lane). APTX (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain reacted with APTX Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
APTX Antibody (Center) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | APTX Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APTX; AXA1; Aprataxin; Forkhead-associated domain histidine triad-like protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AXA1 |
|---|---|
| Alternative Names | APTX AXA1 Aprataxin Forkhead-associated domain histidine triad-like protein |
| Molecular Weight(MW) | 40740 Da |
| Function | DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non- ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined. Also able to hydrolyze adenosine 5'- monophosphoramidate (AMP-NH(2)) and diadenosine tetraphosphate (AppppA), but with lower catalytic activity |
| Tissue Specificity | Widely expressed. In brain, it is expressed in the posterior cortex, cerebellum, hippocampus and olfactory bulb Isoform 1 is highly expressed in the cerebral cortex and cerebellum, compared to isoform 2 |
| Cellular Localization | Nucleus, nucleoplasm. Nucleus, nucleolus. Note=Upon genotoxic stress, colocalizes with XRCC1 at sites of DNA damage. Colocalizes with MDC1 at sites of DNA double-strand breaks. Interaction with NCL is required for nucleolar localization |
| Entrez Gene | 54840 |
|---|
Application
Western blot analysis of APTX Antibody (Center) in HepG2 cell line lysates (35ug/lane). APTX (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain reacted with APTX Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
APTX Antibody (Center) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
