Application
Western blot analysis of ARF6 Antibody (C-term) in T47D,MCF-7 cell line lysates (35ug/lane). ARF6 (arrow) was detected using the purified Pab.(2ug/ml)Application
Western blot analysis of ARF6 (arrow) using rabbit polyclonal ARF6 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ARF6 gene (Lane 2) .Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ARF6 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
ARF6 Antibody (C-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ARF6 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARF6; ADP-ribosylation factor 6 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ARF6 ADP-ribosylation factor 6 |
|---|---|
| Molecular Weight(MW) | 20082 Da |
| Function | GTP-binding protein involved in protein trafficking; regulates endocytic recycling and cytoskeleton remodeling. May modulate vesicle budding and uncoating within the Golgi apparatus Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in the regulation of dendritic spine development (By similarity) Contributes to the regulation of dendritic branching and filopodia extension |
| Tissue Specificity | Ubiquitous, with higher levels in heart, substantia nigra, and kidney |
| Cellular Localization | Golgi apparatus. Cell membrane; Lipid- anchor. Endosome membrane; Lipid-anchor. Cell membrane. Cell projection, filopodium membrane; Lipid-anchor. Note=Recruited to the cell membrane in association with CYTH2 and ARL4C |
| Entrez Gene | 382 |
|---|
Application
Western blot analysis of ARF6 Antibody (C-term) in T47D,MCF-7 cell line lysates (35ug/lane). ARF6 (arrow) was detected using the purified Pab.(2ug/ml)
Application
Western blot analysis of ARF6 (arrow) using rabbit polyclonal ARF6 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ARF6 gene (Lane 2) .
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ARF6 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
ARF6 Antibody (C-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
