Application
A. Western blot analysis on 293 cell lysate using anti-ARF1 RabMAb (Cat. #AJ1060a), dilution 1:2,000.Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-ARF1 RabMAb (Cat. #AJ1060a).| Product Name | ARF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARF1, ADP-ribosylation factor 1 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP442Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:2000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ARF1 ADP-ribosylation factor 1 |
|---|---|
| Molecular Weight(MW) | 20697 Da |
| Function | GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles |
| Tissue Specificity | A synthetic peptide corresponding to residues in human ARF1 was used as immunogen. This antibody is predicted to detect splice isoforms 3, 4, 5 based on sequence analysis. |
| Cellular Localization | Golgi apparatus. Cytoplasm, perinuclear region |
| Entrez Gene | 375 |
|---|
Application
A. Western blot analysis on 293 cell lysate using anti-ARF1 RabMAb (Cat. #AJ1060a), dilution 1:2,000.
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-ARF1 RabMAb (Cat. #AJ1060a).| Application Notes | WB:1:2000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
