Application
ARF1 Antibody (Center) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the ARF1 antibody detected the ARF1 protein (arrow).Application
ARF1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ARF1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | ARF1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARF1; ADP-ribosylation factor 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ARF1 ADP-ribosylation factor 1 |
|---|---|
| Molecular Weight(MW) | 20697 Da |
| Function | GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles |
| Tissue Specificity | This ARF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 87-114 amino acids from the Central region of human ARF1. |
| Cellular Localization | Golgi apparatus. Cytoplasm, perinuclear region |
| Entrez Gene | 375 |
|---|---|
| Nucleotide Accession | NP_001019398.1 NP_001649.1 NP_001019399.1 NP_001019397.1 |
Application
ARF1 Antibody (Center) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the ARF1 antibody detected the ARF1 protein (arrow).
Application
ARF1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ARF1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
