Application
Western blot analysis of LDLRAP1 Antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). LDLRAP1 (arrow) was detected using the purified Pab (1:60 dilution).Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with LDLRAP1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | ARH (LDLRAP1) Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LDLRAP1; ARH; Low density lipoprotein receptor adapter protein 1; Autosomal recessive hypercholesterolemia protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ARH |
|---|---|
| Alternative Names | LDLRAP1 ARH Low density lipoprotein receptor adapter protein 1 Autosomal recessive hypercholesterolemia protein |
| Molecular Weight(MW) | 33885 Da |
| Function | Adapter protein (clathrin-associated sorting protein (CLASP)) required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). May be required for LDL binding and internalization but not for receptor clustering in coated pits. May facilitate the endocytocis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits. May also be involved in the internalization of other LDLR family members Binds to phosphoinositides, which regulate clathrin bud assembly at the cell surface |
| Tissue Specificity | Expressed at high levels in the kidney, liver, and placenta, with lower levels detectable in brain, heart, muscle, colon, spleen, intestine, lung, and leukocytes |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 26119 |
|---|
Application
Western blot analysis of LDLRAP1 Antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). LDLRAP1 (arrow) was detected using the purified Pab (1:60 dilution).
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with LDLRAP1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
