Application
ARHB Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ARHB antibody detected the ARHB protein (arrow).Application
ARHB Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ARHB Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | ARHB Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RHOB; ARH6; ARHB; Rho-related GTP-binding protein RhoB; Rho cDNA clone 6 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ARH6 ARHB |
|---|---|
| Alternative Names | RHOB ARH6 ARHB Rho-related GTP-binding protein RhoB Rho cDNA clone 6 |
| Molecular Weight(MW) | 22123 Da |
| Function | Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis Required for genotoxic stress-induced cell death in breast cancer cells |
| Tissue Specificity | This ARHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-137 amino acids from the Central region of human ARHB. |
| Cellular Localization | Late endosome membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Nucleus. Cleavage furrow. Note=Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus. Translocates to the equatorial region before furrow formation in a ECT2-dependent manner |
| Entrez Gene | 388 |
|---|---|
| Nucleotide Accession | NP_004031.1 |
Application
ARHB Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ARHB antibody detected the ARHB protein (arrow).
Application
ARHB Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ARHB Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
