Application
ARHG7 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ARHG7 antibody detected the ARHG7 protein (arrow).Application
ARHG7 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ARHG7 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | ARHG7 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARHGEF7; COOL1; KIAA0142; P85SPR; PAK3BP; PIXB; Rho guanine nucleotide exchange factor 7; Beta-Pix; COOL-1; PAK-interacting exchange factor beta; p85 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | COOL1 KIAA0142 P85SPR PAK3BP PIXB |
|---|---|
| Alternative Names | ARHGEF7 COOL1 KIAA0142 P85SPR PAK3BP PIXB Rho guanine nucleotide exchange factor 7 Beta-Pix COOL-1 PAK-interacting exchange factor beta p85 |
| Molecular Weight(MW) | 90012 Da |
| Function | Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions (By similarity). May function as a positive regulator of apoptosis. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons |
| Tissue Specificity | This ARHG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 748-777 amino acids from the C-terminal region of human ARHG7. |
| Cellular Localization | Cell junction, focal adhesion (By similarity). Cell projection, ruffle (By similarity). Cytoplasm, cell cortex (By similarity). Cell projection, lamellipodium (By similarity). Note=Detected at cell adhesions. A small proportion is detected at focal adhesions |
| Entrez Gene | 8874 |
|---|---|
| Nucleotide Accession | NP_003890.1 NP_663788.1 NP_001106984.1 |
Application
ARHG7 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ARHG7 antibody detected the ARHG7 protein (arrow).
Application
ARHG7 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ARHG7 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
