Application
ARHGAP10 Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the ARHGAP10 antibody detected the ARHGAP10 protein (arrow).Application
ARHGAP10 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ARHGAP10 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
ARHGAP10 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ARHGAP10 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARHGAP10; GRAF2; Rho GTPase-activating protein 10; GTPase regulator associated with focal adhesion kinase 2; Graf-related protein 2; Rho-type GTPase-activating protein 10 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GRAF2 |
|---|---|
| Alternative Names | ARHGAP10 GRAF2 Rho GTPase-activating protein 10 GTPase regulator associated with focal adhesion kinase 2 Graf-related protein 2 Rho-type GTPase-activating protein 10 |
| Molecular Weight(MW) | 89375 Da |
| Function | GTPase activator for the small GTPases RhoA and Cdc42 by converting them to an inactive GDP-bound state. Essential for PTKB2 regulation of cytoskeletal organization via Rho family GTPases. Inhibits PAK2 proteolytic fragment PAK-2p34 kinase activity and changes its localization from the nucleus to the perinuclear region. Stabilizes PAK-2p34 thereby increasing stimulation of cell death (By similarity) |
| Tissue Specificity | High levels of expression in heart and skeletal muscle |
| Cellular Localization | Cytoplasm (By similarity). Cytoplasm, perinuclear region (By similarity). Cell membrane (By similarity) Note=Association to cell membrane is dependent on PH domain (By similarity) |
| Entrez Gene | 79658 |
|---|---|
| Nucleotide Accession | NP_078881.3 |
Application
ARHGAP10 Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the ARHGAP10 antibody detected the ARHGAP10 protein (arrow).
Application
ARHGAP10 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ARHGAP10 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
ARHGAP10 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
