Application
ARHGAP17 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ARHGAP17 antibody detected the ARHGAP17 protein (arrow).Application
ARHGAP17 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ARHGAP17 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARHGAP17; RICH1; Rho GTPase-activating protein 17; Rho-type GTPase-activating protein 17; RhoGAP interacting with CIP4 homologs protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RICH1 |
|---|---|
| Alternative Names | ARHGAP17 RICH1 Rho GTPase-activating protein 17 Rho-type GTPase-activating protein 17 RhoGAP interacting with CIP4 homologs protein 1 |
| Molecular Weight(MW) | 95437 Da |
| Function | Rho GTPase-activating protein involved in the maintenance of tight junction by regulating the activity of CDC42, thereby playing a central role in apical polarity of epithelial cells. Specifically acts as a GTPase activator for the CDC42 GTPase by converting it to an inactive GDP-bound state. The complex formed with AMOT acts by regulating the uptake of polarity proteins at tight junctions, possibly by deciding whether tight junction transmembrane proteins are recycled back to the plasma membrane or sent elsewhere. Participates in the Ca(2+)-dependent regulation of exocytosis, possibly by catalyzing GTPase activity of Rho family proteins and by inducing the reorganization of the cortical actin filaments. Acts as a GTPase activitor in vitro for RAC1 |
| Tissue Specificity | Ubiquitously expressed. Expressed at higher level in heart and placenta |
| Cellular Localization | Membrane; Peripheral membrane protein. Cytoplasm. Cell junction, tight junction. Note=Associates with membranes and concentrates at sites of cell-cell contact |
| Entrez Gene | 55114 |
|---|---|
| Nucleotide Accession | NP_001006635.1 |
Application
ARHGAP17 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ARHGAP17 antibody detected the ARHGAP17 protein (arrow).
Application
ARHGAP17 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
