Application
Western blot analysis of ARHGDIA Antibody (C-term) in A375(lane 1),HL-60(lane 2),Ramos(lane 3) cell line lysates (35ug/lane). ARHGDIA (arrow) was detected using the purified Pab.Application
Western blot analysis of ARHGDIA (arrow) using rabbit polyclonal ARHGDIA Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ARHGDIA gene.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ARHGDIA Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
ARHGDIA Antibody (C-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ARHGDIA Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARHGDIA; GDIA1; Rho GDP-dissociation inhibitor 1; Rho-GDI alpha |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GDIA1 |
|---|---|
| Alternative Names | ARHGDIA GDIA1 Rho GDP-dissociation inhibitor 1 Rho-GDI alpha |
| Molecular Weight(MW) | 23207 Da |
| Function | Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. In glioma cells, inhibits cell migration and invasion by mediating the signals of SEMA5A and PLXNB3 that lead to inactivation of RAC1 (By similarity) |
| Tissue Specificity | This ARHGDIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119~148 amino acids from the C-terminal region of human ARHGDIA. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 396 |
|---|
Application
Western blot analysis of ARHGDIA Antibody (C-term) in A375(lane 1),HL-60(lane 2),Ramos(lane 3) cell line lysates (35ug/lane). ARHGDIA (arrow) was detected using the purified Pab.
Application
Western blot analysis of ARHGDIA (arrow) using rabbit polyclonal ARHGDIA Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ARHGDIA gene.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ARHGDIA Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
ARHGDIA Antibody (C-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
