Application
ARHGAP24 Antibody (C-term) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the ARHGAP24 antibody detected the ARHGAP24 protein (arrow).| Product Name | ARHGAP24 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARHGAP24; FILGAP; Rho GTPase-activating protein 24; Filamin-A-associated RhoGAP; RAC1- and CDC42-specific GTPase-activating protein of 72 kDa; Rho-type GTPase-activating protein 24; RhoGAP of 73 kDa; Sarcoma antigen NY-SAR-88; p73RhoGAP |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FILGAP |
|---|---|
| Alternative Names | ARHGAP24 FILGAP Rho GTPase-activating protein 24 Filamin-A-associated RhoGAP RAC1- and CDC42-specific GTPase-activating protein of 72 kDa Rho-type GTPase-activating protein 24 RhoGAP of 73 kDa Sarcoma antigen NY-SAR-88 p73RhoGAP |
| Molecular Weight(MW) | 84258 Da |
| Function | Rho GTPase-activating protein involved in cell polarity, cell morphology and cytoskeletal organization. Acts as a GTPase activator for the Rac-type GTPase by converting it to an inactive GDP-bound state. Controls actin remodeling by inactivating Rac downstream of Rho leading to suppress leading edge protrusion and promotes cell retraction to achieve cellular polarity. Able to suppress RAC1 and CDC42 activity in vitro. Overexpression induces cell rounding with partial or complete disruption of actin stress fibers and formation of membrane ruffles, lamellipodia, and filopodia. Isoform 2 is a vascular cell-specific GAP involved in modulation of angiogenesis |
| Tissue Specificity | Isoform 1 is widely expressed with a higher level in kidney. Isoform 2 is mainly expressed in endothelial cells |
| Cellular Localization | Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell junction, focal adhesion. Cell projection Note=Localizes to actin stress fibers. In migrating cells, localizes to membrane lamellae and protusions |
| Entrez Gene | 83478 |
|---|---|
| Nucleotide Accession | NP_001020787.2 NP_112595.2 NP_001036134.1 |
Application
ARHGAP24 Antibody (C-term) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the ARHGAP24 antibody detected the ARHGAP24 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
