Application
ARHGEF18 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ARHGEF18 antibody detected the ARHGEF18 protein (arrow).| Product Name | ARHGEF18 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARHGEF18; KIAA0521; Rho guanine nucleotide exchange factor 18; 114 kDa Rho-specific guanine nucleotide exchange factor; Septin-associated RhoGEF |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0521 |
|---|---|
| Alternative Names | ARHGEF18 KIAA0521 Rho guanine nucleotide exchange factor 18 114 kDa Rho-specific guanine nucleotide exchange factor Septin-associated RhoGEF |
| Molecular Weight(MW) | 130780 Da |
| Function | Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. Also act as a GEF for RAC1, inducing production of reactive oxygen species (ROS). Does not act as a GEF for CDC42. The G protein beta-gamma (Gbetagamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change and ROS production |
| Tissue Specificity | Expressed in all tissues tested with highest expression in kidney and pancreas. Weakly or not expressed in liver, skeletal muscle and testis |
| Cellular Localization | Cytoplasm. Note=Colocalizes with actin stress fibers |
| Entrez Gene | 23370 |
|---|---|
| Nucleotide Accession | NP_056133.2 |
Application
ARHGEF18 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ARHGEF18 antibody detected the ARHGEF18 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
