Application
ARID5B Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the ARID5B antibody detected the ARID5B protein (arrow).| Product Name | ARID5B Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARID5B; DESRT; MRF2; AT-rich interactive domain-containing protein 5B; MRF1-like protein; Modulator recognition factor 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DESRT MRF2 |
|---|---|
| Alternative Names | ARID5B DESRT MRF2 AT-rich interactive domain-containing protein 5B MRF1-like protein Modulator recognition factor 2 |
| Molecular Weight(MW) | 132375 Da |
| Function | Transcription coactivator that binds to the 5'-AATA[CT]- 3' core sequence and plays a key role in adipogenesis and liver development. Acts by forming a complex with phosphorylated PHF2, which mediates demethylation at Lys-336, leading to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2- ARID5B complex acts as a coactivator of HNF4A in liver. Required for adipogenesis: regulates triglyceride metabolism in adipocytes by regulating expression of adipogenic genes. Overexpression leads to induction of smooth muscle marker genes, suggesting that it may also act as a regulator of smooth muscle cell differentiation and proliferation. Represses the cytomegalovirus enhancer |
| Tissue Specificity | Widely expressed, including in liver (at protein level) |
| Cellular Localization | Nucleus (By similarity). |
| Entrez Gene | 84159 |
|---|---|
| Nucleotide Accession | NP_115575.1 |
Application
ARID5B Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the ARID5B antibody detected the ARID5B protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
