Application
"Western blot analysis of UbcH7(arrow) using rabbit polyclonal UbcH7-BP Antibody (C-term) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ARIH1 gene (Lane 2) (Origene Technologies)."Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | ARIH1 (UbcH7-BP) Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARIH1; ARI; MOP6; UBCH7BP; E3 ubiquitin-protein ligase ARIH1; H7-AP2; HHARI; Monocyte protein 6; Protein ariadne-1 homolog; UbcH7-binding protein; UbcM4-interacting protein; Ubiquitin-conjugating enzyme E2-binding protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ARI MOP6 UBCH7BP |
|---|---|
| Alternative Names | ARIH1 ARI MOP6 UBCH7BP E3 ubiquitin-protein ligase ARIH1 H7-AP2 HHARI Monocyte protein 6 Protein ariadne-1 homolog UbcH7-binding protein UbcM4-interacting protein Ubiquitin-conjugating enzyme E2-binding protein 1 |
| Molecular Weight(MW) | 64118 Da |
| Function | E3 ubiquitin-protein ligase, which catalyzes polyubiquitination of target proteins together with ubiquitin- conjugating enzyme E2 UBE2L3. May play a role in protein translation by mediating polyubiquitination of EIF4E2, leading to its subsequent degradation |
| Tissue Specificity | Widely expressed. |
| Cellular Localization | Cytoplasm. Note=Mainly cytoplasmic. |
| Entrez Gene | 25820 |
|---|
Application
"Western blot analysis of UbcH7(arrow) using rabbit polyclonal UbcH7-BP Antibody (C-term) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ARIH1 gene (Lane 2) (Origene Technologies)."
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
