Application
ARNT2 Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ARNT2 antibody detected the ARNT2 protein (arrow).Application
Fluorescent confocal image of SK-BR-3 cell stained with ARNT2 Antibody (Center)(Cat#AP16544c). SK-BR-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ARNT2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).ARNT2 immunoreactivity is localized to nucleus significantly.| Product Name | ARNT2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARNT2; BHLHE1; KIAA0307; Aryl hydrocarbon receptor nuclear translocator 2; Class E basic helix-loop-helix protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BHLHE1 KIAA0307 |
|---|---|
| Alternative Names | ARNT2 BHLHE1 KIAA0307 Aryl hydrocarbon receptor nuclear translocator 2 Class E basic helix-loop-helix protein 1 |
| Molecular Weight(MW) | 78691 Da |
| Function | Specifically recognizes the xenobiotic response element (XRE) |
| Tissue Specificity | This ARNT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 248-278 amino acids from the Central region of human ARNT2. |
| Cellular Localization | Nucleus (Potential). |
| Entrez Gene | 9915 |
|---|---|
| Nucleotide Accession | NP_055677.3 |
Application
ARNT2 Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ARNT2 antibody detected the ARNT2 protein (arrow).
Application
Fluorescent confocal image of SK-BR-3 cell stained with ARNT2 Antibody (Center)(Cat#AP16544c). SK-BR-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ARNT2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).ARNT2 immunoreactivity is localized to nucleus significantly.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
