Application
Western blot analysis of ARPC1A Antibody (C-term) in Hela cell line lysates (35ug/lane). ARPC1A (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ARPC1A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Immunofluorescence analysis of ARPC1A Antibody (C-term) with hela cells. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.Application
Flow cytometric analysis of WiDr cells using ARPC1A Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ARPC1A Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARPC1A; SOP2L; Actin-related protein 2/3 complex subunit 1A; SOP2-like protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SOP2L |
|---|---|
| Alternative Names | ARPC1A SOP2L Actin-related protein 2/3 complex subunit 1A SOP2-like protein |
| Molecular Weight(MW) | 41569 Da |
| Function | Probably functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks |
| Tissue Specificity | This ARPC1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 293~323 amino acids from the C-terminal region of human ARPC1A. |
| Cellular Localization | Cytoplasm, cytoskeleton (Probable). |
| Entrez Gene | 10552 |
|---|
Application
Western blot analysis of ARPC1A Antibody (C-term) in Hela cell line lysates (35ug/lane). ARPC1A (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ARPC1A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Immunofluorescence analysis of ARPC1A Antibody (C-term) with hela cells. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
Application
Flow cytometric analysis of WiDr cells using ARPC1A Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
