Application
ASAH2 Antibody (C-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the ASAH2 antibody detected the ASAH2 protein (arrow).| Product Name | ASAH2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ASAH2; HNAC1; Neutral ceramidase; Acylsphingosine deacylase 2; BCDase; LCDase; N-acylsphingosine amidohydrolase 2; Non-lysosomal ceramidase; Neutral ceramidase soluble form |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HNAC1 |
|---|---|
| Alternative Names | ASAH2 HNAC1 Neutral ceramidase Acylsphingosine deacylase 2 BCDase LCDase N-acylsphingosine amidohydrolase 2 Non-lysosomal ceramidase Neutral ceramidase soluble form |
| Molecular Weight(MW) | 85516 Da |
| Function | Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract |
| Tissue Specificity | Primarily expressed in the intestine (PubMed:17334805). Ubiquitously expressed with higher levels in kidney, skeletal muscle and heart (PubMed:10781606). According to PubMed:17334805, ubiquitous expression attributed to ASAH2 may be actually that of the paralog ASAH2B |
| Cellular Localization | Cell membrane; Single-pass type II membrane protein. Note=The neutral ceramidase soluble form is a secreted protein. According to PubMed:10781606, it is mitochondrial However, they used a shorter form in its N-terminus, which may explain this localization which probably does not exist in vivo |
| Entrez Gene | 653308 |
|---|---|
| Nucleotide Accession | NP_001072984.1 |
Application
ASAH2 Antibody (C-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the ASAH2 antibody detected the ASAH2 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
