Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-ATF-1 (pS63) RabMAb (Cat. #AJ1063a), 1:100,000 dilution. Cells were either (A) untreated (B) treated with Lambda Phosphatase.Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-specific anti-ATF-1 RabMAb (Cat. #AJ1063a).| Product Name | ATF-1 Antibody Phospho (pS63) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S63 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1591(2)Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ATF1 Cyclic AMP-dependent transcription factor ATF-1 Activating transcription factor 1 Protein TREB36 |
|---|---|
| Molecular Weight(MW) | 29233 Da |
| Function | This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation |
| Tissue Specificity | A phospho specific peptide corresponding to residues surrounding serine 63 of human ATF-1 was used as an immunogen. This antibody detects ATF-1 phosphorylated on serine 63 |
| Cellular Localization | Nucleus. |
| Entrez Gene | 466 |
|---|
Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-ATF-1 (pS63) RabMAb (Cat. #AJ1063a), 1:100,000 dilution. Cells were either (A) untreated (B) treated with Lambda Phosphatase.
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-specific anti-ATF-1 RabMAb (Cat. #AJ1063a).| Application Notes | WB:1:500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
