Application
Western blot analysis of ATF1 Antibody (C-term) in Jurkat cell line lysates (35ug/lane). ATF1 (arrow) was detected using the purified Pab.Application
ATF1 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ATF1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ATF1 Cyclic AMP-dependent transcription factor ATF-1 Activating transcription factor 1 Protein TREB36 |
|---|---|
| Molecular Weight(MW) | 29233 Da |
| Function | This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation |
| Tissue Specificity | This ATF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ATF1. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 466 |
|---|
Application
Western blot analysis of ATF1 Antibody (C-term) in Jurkat cell line lysates (35ug/lane). ATF1 (arrow) was detected using the purified Pab.
Application
ATF1 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
