Application
Western blot analysis of ATF7 Antibody (N-term) in HL-60 cell line lysates (35ug/lane). ATF7 (arrow) was detected using the purified Pab.Application
ATF7 Antibody (N-term) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ATF7 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100-500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ATFA |
|---|---|
| Alternative Names | ATF7 ATFA Cyclic AMP-dependent transcription factor ATF-7 Activating transcription factor 7 Transcription factor ATF-A |
| Molecular Weight(MW) | 52967 Da |
| Function | Plays important functions in early cell signaling. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]- 3'), a sequence present in many viral and cellular promoters Activator of the NF-ELAM1/delta-A site of the E-selectin promoter Has no intrinsic transcriptional activity, but activates transcription on formation of JUN or FOS heterodimers. Also can bind TRE promoter sequences when heterodimerized with members of the JUN family Isoform 5/ATF-4 acts as a negative regulator, inhibiting both ATF2 and ATF7 transcriptional activities. It may exert these effects by sequestrating in the cytoplasm the Thr-53 phosphorylating kinase, preventing activation |
| Tissue Specificity | Expressed in heart, lung and skeletal muscle. Isoform 4 is expressed in various tissues including heart, brain, placenta, lung and skeletal muscle. Highest levels in skeletal muscle. Lowest in lung and placenta |
| Cellular Localization | Nucleus. Nucleus, nucleoplasm. Note=Mainly nucleoplasmic. Restricted distribution to the perinuculear region The sumoylated form locates to the nuclear periphery |
| Entrez Gene | 11016 |
|---|
Application
Western blot analysis of ATF7 Antibody (N-term) in HL-60 cell line lysates (35ug/lane). ATF7 (arrow) was detected using the purified Pab.
Application
ATF7 Antibody (N-term) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100-500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
