Application
ATF4 Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the ATF4 antibody detected the ATF4 protein (arrow).| Product Name | ATF4 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CREB2, TXREB |
|---|---|
| Alternative Names | ATF4 CREB2 TXREB Cyclic AMP-dependent transcription factor ATF-4 Activating transcription factor 4 Cyclic AMP-responsive element-binding protein 2 DNA-binding protein TAXREB67 Tax-responsive enhancer element-binding protein 67 |
| Molecular Weight(MW) | 38590 Da |
| Function | Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Cooperates with FOXO1 in osteoblasts to regulate glucose homeostasis through suppression of beta-cell production and decrease in insulin production (By similarity). It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I. Regulates the induction of DDIT3/CHOP and asparagine synthetase (ASNS) in response to ER stress. In concert with DDIT3/CHOP, activates the transcription of TRIB3 and promotes ER stress-induced neuronal apoptosis by regulating the transcriptional induction of BBC3/PUMA |
| Tissue Specificity | This ATF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-198 amino acids from the Central region of human ATF4. |
| Cellular Localization | Cytoplasm. Cell membrane. Nucleus. Cytoplasm, cytoskeleton, centrosome. Note=Colocalizes with GABBR1 in hippocampal neuron dendritic membranes (By similarity). Co- localizes with NEK6 in the centrosome |
| Entrez Gene | 468 |
|---|---|
| Nucleotide Accession | NP_001666.2 |
Application
ATF4 Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the ATF4 antibody detected the ATF4 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
