Application
Western blot analysis of ATG14 Antibody (Center) in 293 cell line lysates (35ug/lane).ATG14 (arrow) was detected using the purified Pab.Application
ATG14 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ATG14 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0831 |
|---|---|
| Alternative Names | ATG14 KIAA0831 Beclin 1-associated autophagy-related key regulator Autophagy-related protein 14-like protein |
| Molecular Weight(MW) | 55309 Da |
| Function | Required for both basal and inducible autophagy. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner (By similarity) |
| Tissue Specificity | This ATG14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-200 amino acids from the Central region of human ATG14. |
| Cellular Localization | Cytoplasm. Endoplasmic reticulum. Note=Cytosolic under nutrient-rich conditions. Following autophagy stimuli, such as starvation or rapamycin induction, predominantly detected in cytoplasmic foci, identified as isolation membranes and autophagosomes |
| Entrez Gene | 22863 |
|---|
Application
Western blot analysis of ATG14 Antibody (Center) in 293 cell line lysates (35ug/lane).ATG14 (arrow) was detected using the purified Pab.
Application
ATG14 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
