Application
Western blot analysis of hAPG12L-T23 in MCF-7 cell line lysates (35ug/lane). APG12L (arrow) was detected using the purified Pab.Application
Western blot analysis of hAPG12L-T23 in mouse brain tissue lysates (35ug/lane). APG12L (arrow) was detected using the purified Pab.Application
Mouse leukaemic monocyte macrophage cells treated with vinblastine for 1 hr. APG12L antibody (Cat AP1151a) detected punctuate staining indicative of nascent autophagic vacuole structures. Data courtesy of Dr. Barry Boland, Department of Pharmacology, Oxford University.| Product Name | ATG12 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | APG12, APG12L |
|---|---|
| Alternative Names | ATG12 APG12 APG12L Ubiquitin-like protein ATG12 Autophagy-related protein 12 |
| Molecular Weight(MW) | 15113 Da |
| Function | Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5 |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Cytoplasm (By similarity). |
| Entrez Gene | 9140 |
|---|
Application
Western blot analysis of hAPG12L-T23 in MCF-7 cell line lysates (35ug/lane). APG12L (arrow) was detected using the purified Pab.
Application
Western blot analysis of hAPG12L-T23 in mouse brain tissue lysates (35ug/lane). APG12L (arrow) was detected using the purified Pab.
Application
Mouse leukaemic monocyte macrophage cells treated with vinblastine for 1 hr. APG12L antibody (Cat AP1151a) detected punctuate staining indicative of nascent autophagic vacuole structures. Data courtesy of Dr. Barry Boland, Department of Pharmacology, Oxford University.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
