Application
Western blot analysis of anti-APG12 Monoclonal Antibody (Cat.#AM1816a) by APG12 recombinant protein. APG12(arrow) was detected using the purified Mab.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue and lung carcinoma tissue reacted with APG12L Monoclonal Antibody (Cat.#AM1816a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with APG12L Monoclonal Antibody (Cat.#AM1816a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Atg12 antibody tested in HEK cells. Fixed in PFA permeablilized with 0.2% Saponin. Blocked and antibodies in 10% goat serum. Secondary was mouse 555. The right is in full medium (FM) and the left with EBSS (no leupeptin)(Provided by Nicole McKnight & Sharon Tooze).| Product Name | ATG12 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Clone Number | 43CT73.3.5.5.4 |
| Host Species | Mouse |
| Tested Applications | WBIHCIF |
| WB:1:200~2000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | APG12, APG12L |
|---|---|
| Alternative Names | ATG12 APG12 APG12L Ubiquitin-like protein ATG12 Autophagy-related protein 12 |
| Molecular Weight(MW) | 15113 Da |
| Function | Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5 |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Cytoplasm (By similarity). |
| Entrez Gene | 9140 |
|---|
Application
Western blot analysis of anti-APG12 Monoclonal Antibody (Cat.#AM1816a) by APG12 recombinant protein. APG12(arrow) was detected using the purified Mab.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue and lung carcinoma tissue reacted with APG12L Monoclonal Antibody (Cat.#AM1816a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with APG12L Monoclonal Antibody (Cat.#AM1816a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Atg12 antibody tested in HEK cells. Fixed in PFA permeablilized with 0.2% Saponin. Blocked and antibodies in 10% goat serum. Secondary was mouse 555. The right is in full medium (FM) and the left with EBSS (no leupeptin)(Provided by Nicole McKnight & Sharon Tooze).| Application Notes | WB:1:200~2000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
