Application
Western blot analysis of hAPG16L-K366 in NIH-3T3, HepG2, Hela, Jurkat and NCI-H460 cell line lysates (35ug/lane). APG16L (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with Autophagy APG16L antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | ATG16L Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | APG16L |
|---|---|
| Alternative Names | ATG16L1 APG16L Autophagy-related protein 16-1 APG16-like 1 |
| Molecular Weight(MW) | 68265 Da |
| Function | Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II |
| Tissue Specificity | This ATG16L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 377~407 amino acids from the C-term of human APG16L. |
| Cellular Localization | Cytoplasm (By similarity). Preautophagosomal structure membrane; Peripheral membrane protein (By similarity) Note=Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5 (By similarity) |
| Entrez Gene | 55054 |
|---|
Application
Western blot analysis of hAPG16L-K366 in NIH-3T3, HepG2, Hela, Jurkat and NCI-H460 cell line lysates (35ug/lane). APG16L (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with Autophagy APG16L antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
