Application
Western blot analysis of anti-APG4D Pab in mouse kidney tissue lysate. APG4D(arrow) was detected using the purified Pab.Application
Western blot analysis of anti-hAPG4D-L235 Pab in 293 cell line lysates transiently transfected with the APG4D gene (2ug/lane). hAPG4D-L235(arrow) was detected using the purified Pab (1:60 dilution).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.Application
APG4D Antibody (L235) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the APG4D antibody detected the APG4D protein (arrow).| Product Name | ATG4D Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | APG4D, AUTL4 |
|---|---|
| Alternative Names | ATG4D APG4D AUTL4 Cysteine protease ATG4D AUT-like 4 cysteine endopeptidase Autophagin-4 Autophagy-related cysteine endopeptidase 4 Autophagy-related protein 4 homolog D |
| Molecular Weight(MW) | 52922 Da |
| Function | Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes |
| Tissue Specificity | Mainly expressed in skeletal muscle and, to a lower extent, in testis |
| Cellular Localization | Cytoplasm (Probable). |
| Entrez Gene | 84971 |
|---|
Application
Western blot analysis of anti-APG4D Pab in mouse kidney tissue lysate. APG4D(arrow) was detected using the purified Pab.
Application
Western blot analysis of anti-hAPG4D-L235 Pab in 293 cell line lysates transiently transfected with the APG4D gene (2ug/lane). hAPG4D-L235(arrow) was detected using the purified Pab (1:60 dilution).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Application
APG4D Antibody (L235) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the APG4D antibody detected the APG4D protein (arrow).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
