Application
A. Western blot analysis on HEK293 cell lysates using anti-ATM Phospho (pS1981) RabMAb (Cat. #AJ1066b), dilution at 1:10,000. Cells were either (A) untreated or (B) treated with doxorubicin.Application
B. Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using anti-ATM Phospho (pS1981) RabMAb (Cat. #AJ1066b).| Product Name | ATM Antibody Phospho (pS1981) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S1981 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1890Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:5000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ATM Serine-protein kinase ATM Ataxia telangiectasia mutated |
|---|---|
| Molecular Weight(MW) | 350714 Da |
| Function | Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK Phosphorylates DYRK2, CHEK2, p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation |
| Tissue Specificity | Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes |
| Cellular Localization | Nucleus. Cytoplasmic vesicle. Note=Primarily nuclear. Found also in endocytic vesicles in association with beta-adaptin |
| Entrez Gene | 472 |
|---|
Application
A. Western blot analysis on HEK293 cell lysates using anti-ATM Phospho (pS1981) RabMAb (Cat. #AJ1066b), dilution at 1:10,000. Cells were either (A) untreated or (B) treated with doxorubicin.
Application
B. Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using anti-ATM Phospho (pS1981) RabMAb (Cat. #AJ1066b).| Application Notes | WB:1:5000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
