A. Western blot analysis on 3T3 cell lysates using anti-Phospho-ACL (pS455) RabMAb (Cat. #AJ1067b), 1:5000 dilution. Cells were either (A) untreated (B) treated with insulin.
ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine
Tissue Specificity
A synthetic peptide corresponding to residues of human ATP citrate lyase was used as immunogen.
A. Western blot analysis on 3T3 cell lysates using anti-Phospho-ACL (pS455) RabMAb (Cat. #AJ1067b), 1:5000 dilution. Cells were either (A) untreated (B) treated with insulin.
Application Notes
WB:1:5000
Additional Information
Form
Liquid
Storage Instructions
For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.