Application
A. Western blot analysis on 3T3 cell lysates using anti-Phospho- ATP citrate lyase (pT447/pS451) RabMAb (Cat. #AJ1067c). Cells were either (A) untreated (B) treated with insulin.Application
B. Immunofluorescent staining of HeLa cells using anti-phosho-ATP citrate lyase RabMAb (Cat. #AJ1067c).| Product Name | ATP-citrate lyase Antibody Phospho (pT447/pS451) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-T447/p-S451 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP737Y |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:5000~20000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ACLY ATP-citrate synthase ATP-citrate (pro-S-)-lyase Citrate cleavage enzyme |
|---|---|
| Molecular Weight(MW) | 120839 Da |
| Function | ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine |
| Tissue Specificity | A synthetic phospho-peptide corresponding to residues surrounding Thr447 and Ser451 of human ATP-citrate (pro-S) lyase was used as immunogen. The antibody only detects ATP-citrate (pro-S) lyase phosphorylated on Threonine 447 and Serine 451. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 47 |
|---|
Application
A. Western blot analysis on 3T3 cell lysates using anti-Phospho- ATP citrate lyase (pT447/pS451) RabMAb (Cat. #AJ1067c). Cells were either (A) untreated (B) treated with insulin.
Application
B. Immunofluorescent staining of HeLa cells using anti-phosho-ATP citrate lyase RabMAb (Cat. #AJ1067c).| Application Notes | WB:1:5000~20000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
