Application
Western blot analysis of ATP12A Antibody (Center) in K562 cell line lysates (35ug/lane). ATP12A (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human skin reacted with ATP12A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
ATP12A Antibody (Center) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ATP12A Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ATP1AL1 |
|---|---|
| Alternative Names | ATP12A ATP1AL1 Potassium-transporting ATPase alpha chain 2 Non-gastric H(+)/K(+) ATPase subunit alpha Proton pump |
| Molecular Weight(MW) | 115511 Da |
| Function | Catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane Responsible for potassium absorption in various tissues |
| Tissue Specificity | Found in skin and kidney. Detected in prostate basal cells (at protein level). Expression is increased in benign prostate hyperplasia and tumor tissues (at protein level) |
| Cellular Localization | Membrane; Multi-pass membrane protein. |
| Entrez Gene | 479 |
|---|
Application
Western blot analysis of ATP12A Antibody (Center) in K562 cell line lysates (35ug/lane). ATP12A (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human skin reacted with ATP12A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
ATP12A Antibody (Center) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
