Application
Western blot analysis of ATP2A1 Antibody (C-term) in K562 cell line lysates (35ug/lane). ATP2A1 (arrow) was detected using the purified Pab.Application
ATP2A1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP2A1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
ATP2A1 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ATP2A1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ATP2A1 Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 Calcium pump 1 Calcium-transporting ATPase sarcoplasmic reticulum type fast twitch skeletal muscle isoform Endoplasmic reticulum class 1/2 Ca(2+) ATPase |
|---|---|
| Molecular Weight(MW) | 110252 Da |
| Function | This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction |
| Tissue Specificity | Skeletal muscle, fast twitch muscle (type II) fibers |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein. Sarcoplasmic reticulum membrane; Multi-pass membrane protein |
| Entrez Gene | 487 |
|---|
Application
Western blot analysis of ATP2A1 Antibody (C-term) in K562 cell line lysates (35ug/lane). ATP2A1 (arrow) was detected using the purified Pab.
Application
ATP2A1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP2A1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
ATP2A1 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
