Application
ATP5A1 Antibody (C-term) western blot analysis in WiDr,NCI-H460,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the ATP5A1 antibody detected the ATP5A1 protein (arrow).Application
ATP5A1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP5A1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | ATP5A1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ATP5A, ATP5AL2, ATPM |
|---|---|
| Alternative Names | ATP5A1 ATP5A ATP5AL2 ATPM ATP synthase subunit alpha mitochondrial |
| Molecular Weight(MW) | 59751 Da |
| Function | Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (By similarity) |
| Tissue Specificity | Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord |
| Cellular Localization | Mitochondrion inner membrane. Cell membrane; Peripheral membrane protein; Extracellular side. Note=Colocalizes with HRG on the cell surface of T-cells |
| Entrez Gene | 498 |
|---|
Application
ATP5A1 Antibody (C-term) western blot analysis in WiDr,NCI-H460,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the ATP5A1 antibody detected the ATP5A1 protein (arrow).
Application
ATP5A1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP5A1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
