Application
ATP5H antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP5H antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
ATP5H Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
ATP5H Antibody (Center) western blot analysis in HepG2,NCI-H460,MDA-MB453 cell line lysates (35ug/lane).This demonstrates the ATP5H antibody detected the ATP5H protein (arrow).| Product Name | ATP5H Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCFCWB |
| IHC:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ATP5H ATP synthase subunit d mitochondrial |
|---|---|
| Molecular Weight(MW) | 18491 Da |
| Function | Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements |
| Tissue Specificity | This ATP5H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-105 amino acids from the Central region of human ATP5H. |
| Cellular Localization | Mitochondrion. Mitochondrion inner membrane. |
| Entrez Gene | 10476 |
|---|---|
| Nucleotide Accession | NP_001003785.1 NP_006347.1 |
Application
ATP5H antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP5H antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
ATP5H Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
ATP5H Antibody (Center) western blot analysis in HepG2,NCI-H460,MDA-MB453 cell line lysates (35ug/lane).This demonstrates the ATP5H antibody detected the ATP5H protein (arrow).| Application Notes | IHC:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
