Application
ATP5J2 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the ATP5J2 antibody detected the ATP5J2 protein (arrow).Application
ATP5J2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP5J2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | ATP5J2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ATP5JL |
|---|---|
| Alternative Names | ATP5J2 ATP5JL ATP synthase subunit f mitochondrial |
| Molecular Weight(MW) | 10918 Da |
| Function | Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane |
| Tissue Specificity | This ATP5J2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-56 amino acids from the Central region of human ATP5J2. |
| Cellular Localization | Mitochondrion. Mitochondrion inner membrane; Single-pass membrane protein (Potential) |
| Entrez Gene | 9551 |
|---|---|
| Nucleotide Accession | NP_004880.1 |
Application
ATP5J2 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the ATP5J2 antibody detected the ATP5J2 protein (arrow).
Application
ATP5J2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP5J2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
