Application
Western blot analysis of ATP6V0A1 Antibody (N-term) in K562 cell line lysates (35ug/lane).ATP6V0A1 (arrow) was detected using the purified Pab.Application
ATP6V0A1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ATP6V0A1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
ATP6V0A1 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ATP6V0A1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ATP6N1, ATP6N1A, VPP1 |
|---|---|
| Alternative Names | ATP6V0A1 ATP6N1 ATP6N1A VPP1 V-type proton ATPase 116 kDa subunit a isoform 1 Clathrin-coated vesicle/synaptic vesicle proton pump 116 kDa subunit Vacuolar adenosine triphosphatase subunit Ac116 Vacuolar proton pump subunit 1 Vacuolar proton transl |
| Molecular Weight(MW) | 96413 Da |
| Function | Required for assembly and activity of the vacuolar ATPase. Potential role in differential targeting and regulation of the enzyme for a specific organelle (By similarity) |
| Tissue Specificity | This ATP6V0A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-80 amino acids from the N-terminal region of human ATP6V0A1. |
| Cellular Localization | Cytoplasmic vesicle membrane; Multi-pass membrane protein. Melanosome. Note=Coated vesicle. Identified by mass spectrometry in melanosome fractions from stage I to stage IV |
| Entrez Gene | 535 |
|---|
Application
Western blot analysis of ATP6V0A1 Antibody (N-term) in K562 cell line lysates (35ug/lane).ATP6V0A1 (arrow) was detected using the purified Pab.
Application
ATP6V0A1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ATP6V0A1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
ATP6V0A1 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
