Application
Western blot analysis of ATP7B antibody (C-term) (Cat.# AP6504b) in HepG2 cell line lysates (35ug/lane). ATP7B (arrow) was detected using the purified Pab.Application
ATP7B Antibody (C-term) (Cat.# AP6504b) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP7B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of ATP7B Antibody (C-term)(Cat#AP6504b) with HepG2 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
ATP7B Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ATP7B Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PWD, WC1, WND |
|---|---|
| Alternative Names | ATP7B PWD WC1 WND Copper-transporting ATPase 2 Copper pump 2 Wilson disease-associated protein WND/140 kDa |
| Molecular Weight(MW) | 157263 Da |
| Function | Involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile |
| Tissue Specificity | Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver The cleaved form WND/140 kDa is found in liver cell lines and other tissues |
| Cellular Localization | Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein (By similarity) Note=Predominantly found in the trans-Golgi network (TGN). Not redistributed to the plasma membrane in response to elevated copper levels WND/140 kDa: Mitochondrion. |
| Entrez Gene | 540 |
|---|
Application
Western blot analysis of ATP7B antibody (C-term) (Cat.# AP6504b) in HepG2 cell line lysates (35ug/lane). ATP7B (arrow) was detected using the purified Pab.
Application
ATP7B Antibody (C-term) (Cat.# AP6504b) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP7B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of ATP7B Antibody (C-term)(Cat#AP6504b) with HepG2 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
ATP7B Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
