Application
Western blot analysis of ATXN2 Antibody (Center) in HepG2 cell line lysates (35ug/lane). ATXN2 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with ATXN2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
ATXN2 Antibody (Center) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ATXN2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ATX2, SCA2, TNRC13 |
|---|---|
| Alternative Names | ATXN2 ATX2 SCA2 TNRC13 Ataxin-2 Spinocerebellar ataxia type 2 protein Trinucleotide repeat-containing gene 13 protein |
| Molecular Weight(MW) | 140283 Da |
| Function | Involved in EGFR trafficking, acting as negative regulator of endocytic EGFR internalization at the plasma membrane |
| Tissue Specificity | Expressed in the brain, heart, liver, skeletal muscle, pancreas and placenta. Isoform 1 is predominant in the brain and spinal cord. Isoform 4 is more abundant in the cerebellum. In the brain, broadly expressed in the amygdala, caudate nucleus, corpus callosum, hippocampus, hypothalamus, substantia nigra, subthalamic nucleus and thalamus |
| Cellular Localization | Cytoplasm (By similarity). |
| Entrez Gene | 6311 |
|---|
Application
Western blot analysis of ATXN2 Antibody (Center) in HepG2 cell line lysates (35ug/lane). ATXN2 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with ATXN2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
ATXN2 Antibody (Center) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
