Application
A. Western blot analysis on HepG2 lysate using anti-Aurora C RabMAb (Cat. #AJ1070a), dilution 1:1000| Product Name | Aurora-C Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1011Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AIE2, AIK3, AIRK3, ARK3, STK13 |
|---|---|
| Alternative Names | AURKC AIE2 AIK3 STK13 Serine/threonine-protein kinase 13 Aurora/Ipl1/Eg2 protein 2 Aurora/Ipl1-related kinase 3 Aurora-C |
| Molecular Weight(MW) | 35591 Da |
| Function | Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Plays also a role in meiosis and more particularly in spermatogenesis. Has redundant cellular functions with AURKB and can rescue an AURKB knockdown. Like AURKB, AURKC phosphorylates histone H3 at 'Ser-10' and 'Ser-28' Phosphorylates TACC1, another protein involved in cell division, at 'Ser-228' |
| Tissue Specificity | Isoform 1 and isoform 2 are expressed in testis. Elevated expression levels were seen only in a subset of cancer cell lines such as Hep-G2, Huh-7 and HeLa. Expression is maximum at M phase |
| Cellular Localization | Nucleus. Chromosome. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle. Note=Distributes in the condensed chromosomes during prophase to metaphase. After entering anaphase, there is a dissociation from separated chromosomes and a redistribution to midzone microtubules, and finally remains in the midbody during cytokinesis |
| Entrez Gene | 6795 |
|---|
Application
A. Western blot analysis on HepG2 lysate using anti-Aurora C RabMAb (Cat. #AJ1070a), dilution 1:1000| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
