Application
Figure 1: Western blot analysis using anti-AXL monoclonal antibody against extracellular domain of human AXL(aa19-444).Application
Figure 2: Confocal immunofluorescence analysis of methanol-fixed HEK293 cells trasfected with AXL-hIgGFc using anti-AXL monoclonal antioby(green), showing cytoplasmic and membrane localization. Blue: DRAQ5 fluorescent DNA dye.| Product Name | AXL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 70000000000 |
| Host Species | Mouse |
| Tested Applications | WBIF |
| WB:1:500 - 2000. IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | UFO |
|---|---|
| Alternative Names | UFO JTK11 |
| Molecular Weight(MW) | 98336 Da |
| Function | Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, ALX binds and induces tyrosine phosphorylation of PI3- kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TENC1. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response. In case of filovirus infection, seems to function as a cell entry factor |
| Tissue Specificity | Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein |
| Entrez Gene | 558 |
|---|---|
| Nucleotide Accession | NP_068713.2 |
Application
Figure 1: Western blot analysis using anti-AXL monoclonal antibody against extracellular domain of human AXL(aa19-444).
Application
Figure 2: Confocal immunofluorescence analysis of methanol-fixed HEK293 cells trasfected with AXL-hIgGFc using anti-AXL monoclonal antioby(green), showing cytoplasmic and membrane localization. Blue: DRAQ5 fluorescent DNA dye.| Application Notes | WB:1:500 - 2000. IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant extracellular fragment of human AXL fused with hIgGFc tag expressed in HEK293 cell line. |
