B3GAT3 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the B3GAT3 antibody detected the B3GAT3 protein (arrow).
Glycosaminoglycans biosynthesis. Involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. Transfers a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcUA) to the common linkage region trisaccharide Gal-beta-1,3-Gal-beta-1,4-Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans. Can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins. Shows strict specificity for Gal-beta-1,3-Gal-beta-1,4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc
Tissue Specificity
Ubiquitous (but weakly expressed in all tissues examined)
Cellular Localization
Golgi apparatus membrane; Single-pass type II membrane protein. Golgi apparatus, cis-Golgi network
B3GAT3 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the B3GAT3 antibody detected the B3GAT3 protein (arrow).
Application Notes
WB:1:100~500
Additional Information
Form
Liquid
Storage Instructions
For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.