Application
Western blot analysis on A) fetal spleen B) fetal thymus, C) SKBR-3 lysates using anti-B7-H4 RabMAb (Cat. #AJ1073a), 1:10,000 dilution.Application
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-B7-H4 RabMAb (Cat. #AJ1073a)| Product Name | B7-H4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1165 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | B7H4 |
|---|---|
| Molecular Weight(MW) | 30878 Da |
| Function | Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor- associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation |
| Tissue Specificity | Overexpressed in breast, ovarian, endometrial, renal cell (RCC) and non-small-cell lung cancers (NSCLC) Expressed on activated T- and B-cells, monocytes and dendritic cells, but not expressed in most normal tissues (at protein level). Widely expressed, including in kidney, liver, lung, ovary, placenta, spleen and testis |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein (Potential). Note=Expressed at the cell surface. A soluble form has also been detected |
| Entrez Gene | 79679 |
|---|
Application
Western blot analysis on A) fetal spleen B) fetal thymus, C) SKBR-3 lysates using anti-B7-H4 RabMAb (Cat. #AJ1073a), 1:10,000 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-B7-H4 RabMAb (Cat. #AJ1073a)| Application Notes | WB:1:500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
