Application
A. Western blot analysis on HeLa cell lysate using anti-Bag-1 RabMAb (Cat. #AJ1075a). Dilution 1:500Application
B. Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using anti-Bag-1 RabMAb (Cat. #AJ1075a).Application
C. Immunofluorescent stsining of HeLa cells using anti-Bag-1 RabMAb (Cat. #AJ1075a).| Product Name | Bag-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | Y166 |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HAP |
|---|---|
| Alternative Names | BAG1 HAP BAG family molecular chaperone regulator 1 Bcl-2-associated athanogene 1 Glucocorticoid receptor-associated protein RAP46 |
| Molecular Weight(MW) | 38779 Da |
| Function | Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli |
| Tissue Specificity | Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines (at protein level) |
| Cellular Localization | Isoform 1: Nucleus. Cytoplasm. Note=Isoform 1 localizes predominantly to the nucleus Isoform 4: Cytoplasm. Nucleus. Note=Isoform 4 localizes predominantly to the cytoplasm. The cellular background in which it is expressed can influence whether it resides primarily in the cytoplasm or is also found in the nucleus. In the presence of BCL2, localizes to intracellular membranes (what appears to be the nuclear envelope and perinuclear membranes) as well as punctate cytosolic structures suggestive of mitochondria |
| Entrez Gene | 573 |
|---|
Application
A. Western blot analysis on HeLa cell lysate using anti-Bag-1 RabMAb (Cat. #AJ1075a). Dilution 1:500
Application
B. Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using anti-Bag-1 RabMAb (Cat. #AJ1075a).
Application
C. Immunofluorescent stsining of HeLa cells using anti-Bag-1 RabMAb (Cat. #AJ1075a).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
