Application
The anti-BAP1 Pab is used in Western blot to detect BAP1 in SK-BR-3 cell lysate.| Product Name | BAP1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0272 |
|---|---|
| Alternative Names | BAP1 KIAA0272 Ubiquitin carboxyl-terminal hydrolase BAP1 BRCA1-associated protein 1 Cerebral protein 6 |
| Molecular Weight(MW) | 80362 Da |
| Function | Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1 Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'- linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Acts as a tumor suppressor |
| Tissue Specificity | Highly expressed in testis, placenta and ovary. Expressed in breast |
| Cellular Localization | Cytoplasm. Nucleus. Note=Mainly nuclear. Binds to chromatin |
| Entrez Gene | 8314 |
|---|
Application
The anti-BAP1 Pab is used in Western blot to detect BAP1 in SK-BR-3 cell lysate.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
