Application
BANF1 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the BANF1 antibody detected the BANF1 protein (arrow).Application
BANF1 Antibody (Center) (AP14071c)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BANF1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | BANF1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BAF, BCRG1 |
|---|---|
| Alternative Names | BANF1 BAF BCRG1 Barrier-to-autointegration factor Breakpoint cluster region protein 1 |
| Molecular Weight(MW) | 10059 Da |
| Function | Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging. Exploited by retroviruses for inhibiting self- destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase The association of viral DNA with chromatin requires the presence of BAF and EMD |
| Tissue Specificity | Widely expressed. Expressed in colon, brain, heart, kidney, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen and testis. Not detected in thymus and peripheral blood leukocytes |
| Cellular Localization | Nucleus. Cytoplasm. Chromosome. Note=Significantly enriched at the nuclear inner membrane, diffusely throughout the nucleus during interphase and concentrated at the chromosomes during the M-phase. May be included in HIV-1 virions via its interaction with viral GAG polyprotein. The phosphorylated form (by VRK1) shows a cytoplasmic localization |
| Entrez Gene | 8815 |
|---|---|
| Nucleotide Accession | NP_001137457.1 NP_003851.1 |
Application
BANF1 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the BANF1 antibody detected the BANF1 protein (arrow).
Application
BANF1 Antibody (Center) (AP14071c)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BANF1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
