Application
Western blot analysis of BARON antibody (N-term) (Cat.# AP1831a) in MCF-7 cell line lysates (35ug/lane). BARON (arrow) was detected using the purified Pab.Application
Fluorescent image of U251 cells stained with BARON (N-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1831a BARON (N-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). BARON immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.Application
Formalin-fixed and paraffin-embedded human lymph with BARON Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of MDA-231 cells using BARON Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | BARON Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIFIHCFC |
| WB:1:50~100 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | KIAA0226 Run domain Beclin-1 interacting and cystein-rich containing protein Beclin-1 associated RUN domain containing protein |
|---|---|
| Molecular Weight(MW) | 108622 Da |
| Function | Negatively regulates endocytic trafficking. Impairs the autophagosome maturation process. May inhibit PIK3C3 activity (By similarity) |
| Tissue Specificity | This BARON antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 249~278 amino acids from the N-terminal region of human BARON. |
| Cellular Localization | Late endosome. Lysosome. Early endosome. Note=Predominantly located in late endosomes/lysosomes, only partially detected in early endososome and not at all in the Golgi apparatus |
| Entrez Gene | 9711 |
|---|
Application
Western blot analysis of BARON antibody (N-term) (Cat.# AP1831a) in MCF-7 cell line lysates (35ug/lane). BARON (arrow) was detected using the purified Pab.
Application
Fluorescent image of U251 cells stained with BARON (N-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1831a BARON (N-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). BARON immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
Application
Formalin-fixed and paraffin-embedded human lymph with BARON Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of MDA-231 cells using BARON Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
