Application
BCKDHB Antibody (C-term) western blot analysis in mouse liver tissue lysates (15ug/lane).This demonstrates the BCKDHB antibody detected BCKDHB protein (arrow).Application
BCKDHB Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of BCKDHB Antibody (C-term)(Cat#AP5523b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | BCKDHB Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BCKDHB; 2-oxoisovalerate dehydrogenase subunit beta, mitochondrial; Branched-chain alpha-keto acid dehydrogenase E1 component beta chain |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFCIF |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | BCKDHB 2-oxoisovalerate dehydrogenase subunit beta mitochondrial Branched-chain alpha-keto acid dehydrogenase E1 component beta chain |
|---|---|
| Molecular Weight(MW) | 43122 Da |
| Function | The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3) |
| Tissue Specificity | This BCKDHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 360-390 amino acids from the C-terminal region of human BCKDHB. |
| Cellular Localization | Mitochondrion matrix. |
| Entrez Gene | 594 |
|---|---|
| Nucleotide Accession | NP_000047.1 |
Application
BCKDHB Antibody (C-term) western blot analysis in mouse liver tissue lysates (15ug/lane).This demonstrates the BCKDHB antibody detected BCKDHB protein (arrow).
Application
BCKDHB Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of BCKDHB Antibody (C-term)(Cat#AP5523b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
