Application
The anti-BCKDK Pab is used in Western blot to detect BCKDK in mouse intestine tissue lysate (Lane 1) and Hela cell lysate (Lane 2).Application
Western blot analysis of BCKDK (arrow) using rabbit polyclonal BCKDK Antibody (C-term T340) (RB05257).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BCKDK gene (Lane 2) (Origene Technologies).| Product Name | BCKDK Antibody (C-term T340) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BCKDK; [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial; Branched-chain alpha-ketoacid dehydrogenase kinase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | BCKDK [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase mitochondrial Branched-chain alpha-ketoacid dehydrogenase kinase |
|---|---|
| Molecular Weight(MW) | 46360 Da |
| Function | Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex (By similarity) |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Mitochondrion matrix. |
| Entrez Gene | 10295 |
|---|
Application
The anti-BCKDK Pab is used in Western blot to detect BCKDK in mouse intestine tissue lysate (Lane 1) and Hela cell lysate (Lane 2).
Application
Western blot analysis of BCKDK (arrow) using rabbit polyclonal BCKDK Antibody (C-term T340) (RB05257).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BCKDK gene (Lane 2) (Origene Technologies).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
